In vitro Antiparasitic Activity of Methanolic Extract of Kalanchoe daigremontiana (Crassulaceae) Against Entamoeba histolytica (Amoebida: Entamoebidae) and Trichomonas vaginalis (Trichomonadida: Trichomonadidae)
DOI:
https://doi.org/10.29105/agricolis.v1i1.3Keywords:
Amebosis, Tricomonosis, Hemolysis, Parasitosis, Trichomoniasis, TrophozoitesAbstract
Parasitic infections such as amoebiasis and trichomoniasis represent a significant global public health challenge. Metronidazole as the drug of choice for decades, and its uncontrolled management has led to the emergence of resistant strains. Thus, the need to find new treatments against parasitosis has arisen. Objective: To evaluate the In-vitro antiparasitic activity of the plant Kalanchoe daigremontiana on Entamoeba histolytica and Trichomonas vaginalis. Methods: A methanolic extract of K. daigremontiana was prepared. The extract was qualitatively characterized phytochemically. The effect of the extract on the log-phase trophozoites of E. histolytica and T. vaginalis was determined; finally, its toxicity in human erythrocytes was determined. Results: Phytochemical analysis of K. daigremontiana extract indicated that flavonoids were the most abundant compounds. The extract presented the ability to inhibit the growth of E. histolytica and T. vaginalis with an LD50 of 71 and 105 μg/mL, respectively, and presented low toxicity in erythrocytes. Conclusions: K. daigremontiana extract possesses antiparasitic activity against E. histolytica and T. vaginalis trophozoites. It does not significantly affect red blood cells at effective concentrations against the parasites evaluated.
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